Critical Evaluation of Fully Automated Enzyme Immunoassays for Free Thyroxine and Thyrotropin
نویسندگان
چکیده
منابع مشابه
Performance characteristics of three automated immunoassays for thyroid hormones
Background: Since the introduction of the first radioimmunoassay, several improvements have been made in the design of immunoassays such as method of antibody production, labeling, automation and detection technology. We performed an analytical evaluation of the new electrochemiluminescent immunoassay (ECLIA) for serum TSH, FT4 and T3 in the Elecsys 2010 immunoassay system and compared the resu...
متن کاملHapten-heterologous conjugates evaluated for application to free thyroxine immunoassays.
We evaluated the effect of hapten heterology on free thyroxine (FT4) immunoassays involving the biotin-streptavidin system and time-resolved fluorometry. We compared protein derivatives of thyroxine (T4) and triiodothyronine (T3) as solid-phase antigen or biotinylated protein-tracer conjugate for competitive (or sequential) binding to a mouse anti-T4 monoclonal antibody. In both one- and two-st...
متن کاملEnzyme-amplified immunoassays: a new ultrasensitive assay of thyrotropin evaluated.
An enzyme-amplified immunoassay for thyrotropin has been evaluated. The lower limit of detection of the assay (mean + 3 SD of the zero standard) was 0.037 milli-int.unit/L and the precision was good, giving a working range of 0.13-24.0 milli-int.units/L. The accuracy of the assay was good, as judged from analytical-recovery experiments, analysis of external quality-assessment samples and compar...
متن کاملPediatric reference intervals for serum thyroxine, triiodothyronine, thyrotropin, and free thyroxine.
iodothyronine, Thyrotropin, and Free Thyroxine, David Zurakowski, James Di Canzio, and Joseph A. Majzoub ( Departments of Biostatistics and Medicine and 2 Division of Endocrinology, Department of Medicine, Children’s Hospital, Harvard Medical School, Boston, MA 02115; * address correspondence to this author at: Department of Biostatistics, Children’s Hospital, 300 Longwood Ave., Boston, MA 0211...
متن کاملRadioimmunoassay and automated enzyme immunoassay for the determination of thyroxine.
The EMIT homogeneous enzyme immunoassay for the determination of thyroxine (T4) was adapted to an automatic analyser (Type Kem-O-Mat) and compared with a conventional radioimmunoassay. Sera from 92 patients whose thyrometabolic status was being examined, and control sera with 3 different T4 concentrations were analysed by both methods. Day-to-day analyses of the control sera gave variation coef...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1994
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1994.32.7.549